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human intestinal epithelial cell line caco-2 (BioResource International Inc)

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BioResource International Inc human intestinal epithelial cell line caco-2
Human Intestinal Epithelial Cell Line Caco 2, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+intestinal+epithelial+cell+line+caco-2/caco+2+cells/pm40131980-114-2-11
Average 90 stars, based on 1 article reviews

human intestinal epithelial cell line caco-2 - by Bioz Stars, 2026-09

90/100 stars

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Article Title: Enrichment of Porphyromonas gingivalis in colonic mucosa-associated microbiota and its enhanced adhesion to epithelium in colorectal carcinogenesis: Insights from in vivo and clinical studies.
Article Snippet: The human intestinal epithelial cell line Caco-2 was provided by RIKEN BioResource Center (Tsukuba, Japan).

Article Title: Catechin and quercitrin mitigate the cytotoxic effects of aflatoxin-B1 on liver and colon cells by inhibiting cytochrome P450 1A2 and 3A4, in silico
Article Snippet: Hepatocellular carcinoma and intestinal damage are the most commonly reported issues associated with aflatoxin-B1 (AFB1) intoxications.. AFB1 is biotransformed by cytochrome P450 1A2 (CYP1A2) and 3A4 (CYP3A4) to create a more toxic form, known as AFB1-8,9-epoxide.. This study is aimed to investigate the protective effects of catechin and quercitrin, naturally occurring compounds found in neem leaves (Azadirachta indica), against AFB1-induced cytotoxicity in human liver (HepG2) and colon (Caco-2) cell lines.

Article Title: Selection of Fermentation Supernatant from Probiotic Strains Exhibiting Intestinal Epithelial Barrier Protective Ability and Evaluation of Their Effects on Colitis Mouse and Weaned Piglet Models
Article Snippet: Human intestinal epithelial cell line Caco-2 was obtained from the Bioresource Collection and Research Center, Food Industry Research and Development Institute (Hsinchu, Taiwan), and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, 4.5 g/L D-glucose and L-glutamine, Gibco, Thermo Fisher Scientific Inc., Paisley, Scotland, UK) with or without 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific Inc., Paisley, Scotland, UK), 1% sodium pyruvate (Gibco, Thermo Fisher Scientific Inc., Paisley, Scotland, UK), 1% holo-transferrin (human origin, Sigma, Steinheim, Germany), and antibiotic–antimycotic (Gibco, Thermo Fisher Scientific Inc., Paisley, Scotland, UK) with a regular cell culture protocol.

Article Title: Selection of Fermentation Supernatant from Probiotic Strains Exhibiting Intestinal Epithelial Barrier Protective Ability and Evaluation of Their Effects on Colitis Mouse and Weaned Piglet Models.
Article Snippet: Human intestinal epithelial cell line Caco-2 was obtained from the Bioresource Collection and Research Center, Food Industry Research and Development Institute (Hsinchu, Taiwan), and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, 4.5 g/L Dglucose and L-glutamine, Gibco, Thermo Fisher Scientific Inc., Paisley, Scotland, UK) with or without 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific Inc., Paisley, Scotland, UK), 1% sodium pyruvate (Gibco, Thermo Fisher Scientific Inc., Paisley, Scotland, UK), 1% holo-transferrin (human origin, Sigma, Steinheim, Germany), and antibiotic– Nutrients 2024, 16, 1138 3 of 18 antimycotic (Gibco, Thermo Fisher Scientific Inc., Paisley, Scotland, UK) with a regular cell culture protocol.

Article Title: Candida tropicalis Alters Barrier Permeability and Claudin-1 Organization in Intestinal Epithelial Cells
Article Snippet: The human intestinal epithelial cell line Caco‐2, derived from a colorectal adenocarcinoma and obtained from Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan; No. 60182), was used to model the intestinal epithelium.

Article Title: Anti-inflammatory effects of heat-killed Lactiplantibacillus argentoratensis BBLB001 on a gut inflammation co-culture cell model and dextran sulfate sodium-induced colitis mouse model.
Article Snippet: The human intestinal epithelial cell line Caco-2 and murine macrophage cell line RAW264.7 were purchased from RIKEN BioResource Research Center (Tsukuba, Japan).

Cell Culture:

Article Title: Selection of Fermentation Supernatant from Probiotic Strains Exhibiting Intestinal Epithelial Barrier Protective Ability and Evaluation of Their Effects on Colitis Mouse and Weaned Piglet Models.
Article Snippet: Human intestinal epithelial cell line Caco-2 was obtained from the Bioresource Collection and Research Center, Food Industry Research and Development Institute (Hsinchu, Taiwan), and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, 4.5 g/L Dglucose and L-glutamine, Gibco, Thermo Fisher Scientific Inc., Paisley, Scotland, UK) with or without 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific Inc., Paisley, Scotland, UK), 1% sodium pyruvate (Gibco, Thermo Fisher Scientific Inc., Paisley, Scotland, UK), 1% holo-transferrin (human origin, Sigma, Steinheim, Germany), and antibiotic– Nutrients 2024, 16, 1138 3 of 18 antimycotic (Gibco, Thermo Fisher Scientific Inc., Paisley, Scotland, UK) with a regular cell culture protocol.

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The percentage viability of Caco-2 cells follow 48 h exposure to AA-7 ( a ) and glucose uptake inhibition kinetics with fluorescent glucose 2-NBDG, following treatment with AA-7 ( b ). Data are presented as mean ± SD for three or more independent trials with consistent findings. The use of different letters (a-c) identifies means which exhibit significant differences ( p < 0.05).

Journal: Scientific Reports

Article Title: Bee pollen-derived peptide with dual DPP-IV Inhibition and glucose transport modulation

doi: 10.1038/s41598-026-39009-1

Figure Lengend Snippet: The percentage viability of Caco-2 cells follow 48 h exposure to AA-7 ( a ) and glucose uptake inhibition kinetics with fluorescent glucose 2-NBDG, following treatment with AA-7 ( b ). Data are presented as mean ± SD for three or more independent trials with consistent findings. The use of different letters (a-c) identifies means which exhibit significant differences ( p < 0.05).

Article Snippet: Human intestinal epithelial cell line Caco-2 cells (ATCC ® HTB-37TM) were supplied by the American Type Culture Collection (ATCC, Manassas, VA, USA), before being cultured in Eagle’s Minimum Essential Medium (EMEM) which was supplemented using L-glutamine and 10% fetal bovine serum (Gibco, Rockville, MD, USA).

Techniques: Inhibition

Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).

Article Snippet: Human intestinal epithelial cell lines Caco-2 cells (ATCC: HTB-37) and HT-29 cells (ATCC: HTB-38) were from ATCC and kept in our institute.

Techniques: CCK-8 Assay, Inhibition

NVP-HSP990 significantly inhibited RV replication, viral gene transcription, and antigen expression. ( A ) Caco-2 cells were infected with RV (Wa or SA11 strains) at varying MOIs (0.001–10), followed by treatment with 100 nM NVP-HSP990 or an equal volume of DMSO (as a control) for 24 h. Viral replication was assessed using PFA. ( B ) Schematic diagram illustrating the timing of NVP-HSP990 addition and removal. ( C ) Caco-2 cells were infected with RV (MOI = 1) and treated with 100 nM NVP-HSP990 or with DMSO as control at indicated infection periods as shown in panel B. Viral replication was tested with PFA at 20 h p.i. ( D ) Caco-2 cells were infected with RV (MOI = 1) and treated with 100 nM HSP990 at 0, 4, 8, 12 h p.i. or treated with DMSO at 0 h p.i. Virus replication was tested with PFA at 24 h p.i. ( E ) Caco-2 cells were infected with RV (MOI = 1) and further cultivated with DMEM containing 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the infected cells were harvested for qPCR analysis of the expression of RV genes encoding the VP2, VP6, NSP4, and NSP5 proteins. ( F ) Caco-2 cells were mock-infected with PBS or infected with RV (MOI = 1) and further cultivated with DMEM containing 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the cells were harvested for WB analysis of RV structural proteins VP6 and VP7. ( G ) Caco-2 cells growing on coverslips were mock-infected with PBS or infected with RV (MOI = 3) and treated with 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the infected cells were subjected to immunostaining of RV VP6 antigens (red). Data are presented as mean ± SEM ( A, C, D, E ). The experiments were performed in triplicate ( A, C, D, E ), and the data are representative of two independent experiments ( A, C–G ). ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001 (two-way ANOVA [ A, C, E ] and one-way ANOVA [ D ]).

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: NVP-HSP990 significantly inhibited RV replication, viral gene transcription, and antigen expression. ( A ) Caco-2 cells were infected with RV (Wa or SA11 strains) at varying MOIs (0.001–10), followed by treatment with 100 nM NVP-HSP990 or an equal volume of DMSO (as a control) for 24 h. Viral replication was assessed using PFA. ( B ) Schematic diagram illustrating the timing of NVP-HSP990 addition and removal. ( C ) Caco-2 cells were infected with RV (MOI = 1) and treated with 100 nM NVP-HSP990 or with DMSO as control at indicated infection periods as shown in panel B. Viral replication was tested with PFA at 20 h p.i. ( D ) Caco-2 cells were infected with RV (MOI = 1) and treated with 100 nM HSP990 at 0, 4, 8, 12 h p.i. or treated with DMSO at 0 h p.i. Virus replication was tested with PFA at 24 h p.i. ( E ) Caco-2 cells were infected with RV (MOI = 1) and further cultivated with DMEM containing 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the infected cells were harvested for qPCR analysis of the expression of RV genes encoding the VP2, VP6, NSP4, and NSP5 proteins. ( F ) Caco-2 cells were mock-infected with PBS or infected with RV (MOI = 1) and further cultivated with DMEM containing 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the cells were harvested for WB analysis of RV structural proteins VP6 and VP7. ( G ) Caco-2 cells growing on coverslips were mock-infected with PBS or infected with RV (MOI = 3) and treated with 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the infected cells were subjected to immunostaining of RV VP6 antigens (red). Data are presented as mean ± SEM ( A, C, D, E ). The experiments were performed in triplicate ( A, C, D, E ), and the data are representative of two independent experiments ( A, C–G ). ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001 (two-way ANOVA [ A, C, E ] and one-way ANOVA [ D ]).

Article Snippet: Human intestinal epithelial cell lines Caco-2 cells (ATCC: HTB-37) and HT-29 cells (ATCC: HTB-38) were from ATCC and kept in our institute.

Techniques: Expressing, Infection, Control, Virus, Immunostaining

NVP-HSP990 alters the life state of host cells. Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3) and further cultivated with DMEM containing 100 nM NVP-HSP990 or an equal volume of DMSO as a control for 24 h. Then, the infected cells were harvested for RNA-seq analysis. ( A ) Multiple differential scatter plots of compared groups. ( B ) Venn diagrams of up- and downregulated genes between compared groups. ( C, D ) Top 10 (ranked by descending Q value) upregulated ( C ) and downregulated ( D ) KEGG pathways in all mock-, Wa-, and SA11-infected Caco-2 cells. ( E, F ) Top 10 (ranked by descending Q value) upregulated ( E ) and downregulated ( F ) KEGG pathways in both Wa- and SA11-infected but not in mock-infected Caco-2 cells. *Q value <0.05.

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: NVP-HSP990 alters the life state of host cells. Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3) and further cultivated with DMEM containing 100 nM NVP-HSP990 or an equal volume of DMSO as a control for 24 h. Then, the infected cells were harvested for RNA-seq analysis. ( A ) Multiple differential scatter plots of compared groups. ( B ) Venn diagrams of up- and downregulated genes between compared groups. ( C, D ) Top 10 (ranked by descending Q value) upregulated ( C ) and downregulated ( D ) KEGG pathways in all mock-, Wa-, and SA11-infected Caco-2 cells. ( E, F ) Top 10 (ranked by descending Q value) upregulated ( E ) and downregulated ( F ) KEGG pathways in both Wa- and SA11-infected but not in mock-infected Caco-2 cells. *Q value <0.05.

Article Snippet: Human intestinal epithelial cell lines Caco-2 cells (ATCC: HTB-37) and HT-29 cells (ATCC: HTB-38) were from ATCC and kept in our institute.

Techniques: Infection, Control, RNA Sequencing

NVP-HSP990 inhibited MAPK activation and facilitated expression of tight junction-associated proteins in intestinal cells. ( A ) MA104, Caco-2, and HT-29 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), followed by treatment of 100 nM HSP990 (+) or an equal volume of DMSO as a control (−) for 20 h. Then the infected cells were harvested for WB analysis of MAPK components. ( B ) Caco-2 cells were mock-infected with PBS, treated with 1 µM C16-PAF(C16), or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as a control (−) for 20 h. The infected cells were harvested for WB analysis of MAPK components. ( C ) Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as control (−) for 20 h. Then the infected cells were harvested for WB analysis of tight junction-associated proteins. Data are representative of three ( A ) and two ( B and C ) independent experiments.

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: NVP-HSP990 inhibited MAPK activation and facilitated expression of tight junction-associated proteins in intestinal cells. ( A ) MA104, Caco-2, and HT-29 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), followed by treatment of 100 nM HSP990 (+) or an equal volume of DMSO as a control (−) for 20 h. Then the infected cells were harvested for WB analysis of MAPK components. ( B ) Caco-2 cells were mock-infected with PBS, treated with 1 µM C16-PAF(C16), or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as a control (−) for 20 h. The infected cells were harvested for WB analysis of MAPK components. ( C ) Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as control (−) for 20 h. Then the infected cells were harvested for WB analysis of tight junction-associated proteins. Data are representative of three ( A ) and two ( B and C ) independent experiments.

Article Snippet: Human intestinal epithelial cell lines Caco-2 cells (ATCC: HTB-37) and HT-29 cells (ATCC: HTB-38) were from ATCC and kept in our institute.

Techniques: Activation Assay, Expressing, Infection, Control

NVP-HSP990 mitigated disruption of tight junctions in RV infection. Caco-2 cells growing on coverslips were mock-infected or infected with RV Wa or SA11 strains (MOI = 3), and cultivated with DMEM containing 100 nM NVP-HSP990 or an equal volume of DMSO as a control after infection for another 18 h. Then the infected cells were applied for immunostaining for RV antigens (green), ZO-1 (red), and DAPI staining of nucleus (blue). Data are representative of two independent experiments.

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: NVP-HSP990 mitigated disruption of tight junctions in RV infection. Caco-2 cells growing on coverslips were mock-infected or infected with RV Wa or SA11 strains (MOI = 3), and cultivated with DMEM containing 100 nM NVP-HSP990 or an equal volume of DMSO as a control after infection for another 18 h. Then the infected cells were applied for immunostaining for RV antigens (green), ZO-1 (red), and DAPI staining of nucleus (blue). Data are representative of two independent experiments.

Article Snippet: Human intestinal epithelial cell lines Caco-2 cells (ATCC: HTB-37) and HT-29 cells (ATCC: HTB-38) were from ATCC and kept in our institute.

Techniques: Disruption, Infection, Control, Immunostaining, Staining

Fig. 6 L. reuteri-CMVs promote tight junction proteins in vitro. (A, B) Dil-labeled L. reuteri-CMVs are internalized by HT-29 and Caco2 cells, and the Dil fluorescence intensity increased with prolonged co-incubation time. (C) The mRNA expression levels of ZO-1 and Cdh1 in HT-29 and Caco2 cells. (D-F) The protein levels of ZO-1 and Cdh1 in HT-29 and Caco2 cells using Western blot and IF analysis. Data are mean ± SEM (n = 3). *p < 0.05, **p < 0.01

Journal: Journal of nanobiotechnology

Article Title: Real-world of Limosilactobacillus reuteri in mitigation of acute experimental colitis.

doi: 10.1186/s12951-025-03158-8

Figure Lengend Snippet: Fig. 6 L. reuteri-CMVs promote tight junction proteins in vitro. (A, B) Dil-labeled L. reuteri-CMVs are internalized by HT-29 and Caco2 cells, and the Dil fluorescence intensity increased with prolonged co-incubation time. (C) The mRNA expression levels of ZO-1 and Cdh1 in HT-29 and Caco2 cells. (D-F) The protein levels of ZO-1 and Cdh1 in HT-29 and Caco2 cells using Western blot and IF analysis. Data are mean ± SEM (n = 3). *p < 0.05, **p < 0.01

Article Snippet: HT-29 and Caco2 human intestinal epithelial cell lines were obtained from the American Type Culture Collection (ATCC).

Techniques: In Vitro, Labeling, Fluorescence, Incubation, Expressing, Western Blot

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human intestinal epithelial cell line caco-2 (BioResource International Inc)

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